Kirsty Wark will host the show.
The producers say: "Both are ready to give it their all for BBC Children In Need, but which celebrity team will have the knowledge to finish top of the class?"
The episode was filmed recently, to be broadcast as part of the Children In Need night, which will be on BBC One on Friday 13th November.
Kirsty Wark says: "Hosting University Challenge was the best fun to be had while raising money to give every child who needs it some hope and some care."
Dara O Briain comments: "It's been a dream of mine to be humiliated on University Challenge and now, it's come true!"
Fay Ripley says: "To have the chance to take part in this University Challenge for BBC Children In Need special and to be a team captain for ITV is an honour. BBC Children In Need is a renowned charity and University Challenge is a show I have watched and enjoyed for many years so this is a brilliant experience!"