The BBC says the show will feature "comedy, music and plenty of audience surprises".
The show is due to be recorded as live at the Theatre Royal in Glasgow on the 11th February, for broadcast on the new TV channel's launch night on Sunday 24th February.
Commenting on the gig, Iain Stirling says: "I can't wait to be part of the launch of the new BBC Scotland channel with a big night out at the prestigious Theatre Royal in Glasgow. I spend too much time in London so it'll just be nice to film a TV show where I can speak at a normal pace."
Executive Producer Neil McCallum says: "Hungry Bear Media is delighted to produce A Night At The Theatre as part of the BBC Scotland launch. With the brilliant Iain Stirling overseeing an exciting mix of must-see Scottish comedy and entertainment, we're expecting an unmissable show. A Night At The Theatre is part of our ongoing commitment to growing our production base in Glasgow, which we established over two years ago, and it adds to the work we have done with BBC Scotland through our All Round To Mrs Brown's series."
Gavin Smith, BBC Scotland Commissioning Executive for Scripted and Entertainment, says: "We're thrilled that Iain will be hosting this entertainment extravaganza to kick off our new channel. We want it to bring the fun, warmth and feel-good factor of a big comedy night out at the theatre to living rooms across Scotland."
The new BBC Scotland channel, which will broadcast from 7pm to midnight each day, will be available on Freeview, YouView, Sky, Freesat and Virgin Media, and available via the BBC iPlayer.
The new channel will also show the next and final series of Still Game first, Series 5 of police mocumentary Scot Squad, and has commissioned a comedy drama called Guilt. A TV version of Radio Scotland's panel show Breaking The News will also be shown on the channel, alongside topical comedy series The State Of It and stand-up showcase format The Comedy Underground.
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